The CellScape’s cyclic immunofluorescence workflow begins with a background scan, followed by fully automated staining, washing, and imaging. The cycle ends with gentle but highly effective signal removal using Bruker’s photobleaching-enhancing technology, Epic IFTM, which is compatible with rhodamine, cyanine, and BODIPY family dyes.
Filter Set | Ex/Em | Examples of Compatible Dyes |
FS488 | 482/18 | AlexaFluor488, Atto488, CoraLite488, FITC, Sytox Green |
517/20 | ||
FS532 | 532/18 | AlexaFluor532, Atto532 |
560/14 | ||
FS555 | 550/25 | AlexaFluor555, Atto550, CoraLite555, PE, SparkYG570, Sytox Orange |
605/70 | ||
FS594 | 585/20 | AlexaFluor594, Atto594, CoraLite594, eFluor590 |
623/24 | ||
FS647 | 632/22 | AlexaFluor647, Atto647, CoraLite647, eFluor660 |
694/44 |
Validated Staining Panels for Quantitative Analysis
Bruker’s validated VistaPlex Assays are pre-validated panels compatible with formalin-fixed paraffin-embedded (FFPE) tissues, fresh frozen tissues, and cell suspensions including blood and PBMCs.
Human FFPE | Mouse FFPE | |||
Spatial Immune Profiling | Tissue Architecture | Cell Boundaries | Spatial Immune Profiling | |
CD163 | CD31 | DNA | CD45R/B220 | |
CD19 | CollagenIV | LaminB1 | CD3 | |
CD20 | CD138 | B2M | CD4 | |
CD274/PD-L1 | Podoplanin | ATP1A1 | CD44 | |
CD279/PD-1 | aSMA |
| CD45 | |
CD3 | B-Catenin | CD62L | ||
CD38 | Vimentin | CD138 | ||
CD4 | E-Cadherin | F4/80 | ||
CD45 | CD34 | FoxP3 | ||
CD45RA | Muc1 | Ki67 | ||
CD45RO | CD105 | Ly6C | ||
CD56 | EpCAM | NK1.1 | ||
CD68 |
| PanCK | ||
CD8 | CD279/PD-1 | |||
FoxP3 |
| |||
The Translational Immunology Core is happy to work with investigators to customize/expand these panels! Please email Emma Hornick (emma-hornick@uiowa.edu) for general questions about the CellScape's capabilities or specific project inquiries.