40-color panel to explore major immune cell populations
The following panel provides an in-depth analysis of the activation, differentiation, and chemokine receptor expression of major immune cell subsets including T cells (CD4, CD8, γδ, NKT-like), B cells, dendritic cells, natural killer cells, and monocytes.
Marker | Purpose |
Amine-reactive dye | Viability |
CD45 | Leukocytes |
CD3 | Pan T cell and NKT-like cells |
CD4 | CD4 T cells and NKT-like cells |
CD8 | CD8 T cells, NK cells, and NKT-like cells |
CD25 | Regulatory T cells |
PD-1 | T cell inhibitory receptor |
TCRγδ | Pan γδ T cell |
CD14 | Monocyte differentiation |
CD16 | Monocyte, NK cell, and dendritic cell differentiation |
CD11c | Dendritic cell differentiation |
CD19 | B cells |
CD20 | B cells |
CD24 | B cell differentiation |
CD39 | B cell, regulatory T cell, and monocyte differentiation |
IgD | B cell differentiation |
IgG | B cell differentiation |
IgM | B cell differentiation |
CD141 | Dendritic cell differentiation |
CD1c | Dendritic cells and NKT-like cells |
CD123 | Plasmacytoid dendritic cells |
CD2 | NK cell differentiation |
CD56 | Pan NK cell and γδ T cell activation |
CCR7 | T cell differentiation |
CD27 | T and B cell differentiation |
CD45RA | T cell and dendritic cell differentiation |
CD95 | T and B cell differentiation |
CD127 | Cytokine receptor; T cell differentiation |
CD337 | NK cell differentiation |
CCR6 | Chemokine receptor; T and B cell differentiation |
CCR5 | Chemokine receptor; monocyte, dendritic cell, T cell, and B cell differentiation |
CXCR5 | Chemokine receptor; T cell differentiation |
CXCR3 | Chemokine receptor; dendritic cell, T cell, and B cell differentiation |
HLA-DR | T cell and monocyte activation, NK cell lineage and dendritic cell lineage discrimination |
CD38 | Monocyte, dendritic cell, T cell, and B cell activation/differentiation |
CD57 | NK and CD8 T cell immune senescence |
CD28 | T cell and NK cell differentiation |
CD159a | NK, NKT-like, and γδ T cell activation/differentiation |
CD159c | NK cell differentiation |
CD314 |
NK cell differentiation |
*The above panel was adapted from OMIP-069: Forty-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood