40-color panel to explore major immune cell populations
The following panel provides an in-depth analysis of the activation, differentiation, and chemokine receptor expression of major immune cell subsets including T cells (CD4, CD8, γδ, NKT-like), B cells, dendritic cells, natural killer cells, and monocytes.
| Marker | Purpose |
| Amine-reactive dye | Viability |
| CD45 | Leukocytes |
| CD3 | Pan T cell and NKT-like cells |
| CD4 | CD4 T cells and NKT-like cells |
| CD8 | CD8 T cells, NK cells, and NKT-like cells |
| CD25 | Regulatory T cells |
| PD-1 | T cell inhibitory receptor |
| TCRγδ | Pan γδ T cell |
| CD14 | Monocyte differentiation |
| CD16 | Monocyte, NK cell, and dendritic cell differentiation |
| CD11c | Dendritic cell differentiation |
| CD19 | B cells |
| CD20 | B cells |
| CD24 | B cell differentiation |
| CD39 | B cell, regulatory T cell, and monocyte differentiation |
| IgD | B cell differentiation |
| IgG | B cell differentiation |
| IgM | B cell differentiation |
| CD141 | Dendritic cell differentiation |
| CD1c | Dendritic cells and NKT-like cells |
| CD123 | Plasmacytoid dendritic cells |
| CD2 | NK cell differentiation |
| CD56 | Pan NK cell and γδ T cell activation |
| CCR7 | T cell differentiation |
| CD27 | T and B cell differentiation |
| CD45RA | T cell and dendritic cell differentiation |
| CD95 | T and B cell differentiation |
| CD127 | Cytokine receptor; T cell differentiation |
| CD337 | NK cell differentiation |
| CCR6 | Chemokine receptor; T and B cell differentiation |
| CCR5 | Chemokine receptor; monocyte, dendritic cell, T cell, and B cell differentiation |
| CXCR5 | Chemokine receptor; T cell differentiation |
| CXCR3 | Chemokine receptor; dendritic cell, T cell, and B cell differentiation |
| HLA-DR | T cell and monocyte activation, NK cell lineage and dendritic cell lineage discrimination |
| CD38 | Monocyte, dendritic cell, T cell, and B cell activation/differentiation |
| CD57 | NK and CD8 T cell immune senescence |
| CD28 | T cell and NK cell differentiation |
| CD159a | NK, NKT-like, and γδ T cell activation/differentiation |
| CD159c | NK cell differentiation |
| CD314 |
NK cell differentiation |
*The above panel was adapted from OMIP-069: Forty-Color Full Spectrum Flow Cytometry Panel for Deep Immunophenotyping of Major Cell Subsets in Human Peripheral Blood