Multi-color panels to analyze T cell phenotype and effector function

T cells can be analyzed with or without an in vitro stimulation. In vitro stimulation allows for a more in-depth examination of T cell effector functions.

T cell panel - No stimulation

Marker Purpose
Amine-reactive dye Viability
CD45 Leukocyte marker
CD3 Pan T cell marker
CD4 CD4+ T cell marker
CD8 CD8+ T cell marker
CD25 Regulatory T cell marker
TCRγδ  γδ T cell marker
CD45RA Naïve, stem cell memory, and effector T cell marker
CD45RO T memory cell marker
PD-1 Inhibitory receptor
TIM-3 Inhibitory receptor
CXCR3 Chemokine Receptor
CXCR5 Chemokine Receptor
CX3CR1 Chemokine Receptor
CCR4 Chemokine Receptor
CD127 T cell differentiation
CD56 γδ T cell activation
CCR7 T cell differentiation
CD95 TEMRA differentiation
CD27 Co-stimulatory receptor
CD28 Co-stimulatory receptor
CD226 T cell differentiation
KLRG1 T cell differentiation
HLA-DR Activation marker
CD38 T cell subset differentiation
CD49d Antigen experienced T cell
CD11a Antigen experienced T cell
Foxp3 Regulatory T cell
CD39 T cell differentiation
CD73 T cell differentiation

T cell panel - With stimulation

Marker Purpose
Amine-reactive dye

Viability

CD45 Leukocyte marker
CD3 Pan T cell marker
CD4 CD4+ T cell marker
CD8

CD8+ T cell marker

CD25 Regulatory T cell marker
TCRγδ γδ T cell marker
CD45RA Naive, stem cell memory, and effector T cell marker
CD45RO T cell memory marker
CD137 Regulatory T cell
CCR7 T cell differentiation
CD154 T cell differentiation
IFNγ Effector function
Granzyme B Effector function
TNF Effector function
IL-17A Effector function
IL-21 Effector function
IL-13 Effector function
IL-10 Effector function